Laser Capture Microdissection
LCM offers a method for procuring pure cells from specific microscopic regions of tissue sections and which allows the application of molecular analysis methods to study evolving disease lesions in actual tissue.
Automated, versatie and self-contained Laser Capture Microdissection (LCM) system.
Our new ArcturusXT™ system from MDS Analytical Technologies is a unique microdissection instrument that combines Laser Capture Microdissection (LCM) and Ultraviolet (UV) Laser Cutting in one platform. The open, modular desgin of the ArcturusXT system provides unparalleled research flexibility and versatitity. The system removes the guesswork from the microdissection process by allowing researchers to maintain custody of the sample throughout the experiment, ensuring that only the desired material has been collected.
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Laser Capture Microdissection and UV Laser Cutting
Open, Modular Platform
Simple Intuitive Operation
Superior Image Quality
Sample Custody Maintained at all times
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Visit ArcturusXT at Molecular Dewvices
(http://www.moleculardevices.com/page/instruments/arcturusXT.html) for more information about the ArcturusXT Laser Capture Micodissection system. This web site has all of the information you need to get started. Please watch the video and then register and log into their "Literature and Downloads" section in order to read the following two Protocols before contacting us to use the instrument. Photos and information reproduced curtest of MDS Analytical Technologies.
ArcturusXT Protocols
Tissue Scrape Protocol for Verifying RNA Quality Using the PicoPure™ RNA Isolation Kit Optimized Protocol for Preparing and Staining LCM Samples from Frozen Tissue and Extraction of High-Quality RNA
You will need the following supplies for obtaining RNA from frozen sections.
(if you plan to laser capture from FFPE sections please contact us to discuss your needs)
CapSure HS LCM Caps Starter Pack with
Alignment Tray and Incubation Block
Part Number: LCM0213
PEN Membrane Glass Slides
Part Number: LCM0522
HistroGene®LCM Frozen Section Staining Kit
PicoPure®RMA Isolation Kit
Part Number: KIT0204
Other Useful Links
LCM at NICHD
LCM at NIEHS
NCI/CIT Molecular Profiling Initiative
Other useful links can be found on the Arcturus website.
One Hoppin Street • Providence, RI 02903
Phone: 401-444-2871 • email: esmith6@lifespan.org |
