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Djuro Josic, PhD, is a professor of medicine and pathology at Brown University and director of the Proteomics Core in the COBRE Center for Cancer Research Development. He received his research training at Free University in Berlin, Germany and the National Institutes of Health in Bethesda, Maryland. Josic is internationally known for his expertise in the separation , isolation and identification of biopolymers, particularly membrane and plasma proteins. He has substantial practical experience with and knowledge of a large variety of high throughput, analytical and preparative methods in this filed including HPLC, protein chromatography with monolithic supports, 2-D gel electrophoresis, capillary electrophoresis, affinity purification methodologies, mass spectrometry and similar techniques. The author of more than 140 research articles and book chapters. He was several times the guest editor of the Journal of Chromatography A and is a member of the editorial boards of Electrophoresis and International Journal of Proteomics, and has also reviewed manuscripts for multiple journals. Josic was the chairman of the International Symposium on the Separation of Proteins, peptides, and Polynucleotides (ISPPP’2000) and a member of the scientific committee of ISPPP and the International Symposium of Monoliths. Research focus Purification and isolation of membrane and membrane associated proteins differentially expressed in normal and malignant cells. His current work is focused on characterization of membrane and membrane associate proteins found in the liver and hepatocellular carcinomas. Development of new pharmaceuticals based on human plasma. During his work in industry, Josic was involved in development of several new therapeutics including new preparations of clotting factor VIII, clotting factor IX and antithrombin III. Together with Yow-Pin Lim, MD and Douglas Hixon, PhD, Josic is currently involved in development of virus and immunologically safe preparation of inter-alpha inhibitor proteins from human plasma for treatment of sepsis and other inflammatory diseases. In his last publications, Josic also introduced the use of proteomics in the process development and characterization of so-called ”well characterized biologicals.” Yang, X, Clifton, JC, Huang, F, Kovac, S, Hixson, D, and Josic, Dj, Proteomic analysis for process development and control of therapeutic protein separation from human plasma, Ellectrophoresis (2009) 30, 1185-1193. Huang, F, Clifton, J, Yang, X, Rosenquist, T, Hixson, DC, Kovac, S and Josic, Dj, SELDI-TOF as a method for biomarker discovery in the urine of aristolochic-acid treated mice, Electrophoresis (2009) 30, 1168-1174. Clifton, JG, Huang, F, Kovac, S, Yang, X, Hixson, DC, and Josic, Dj, Proteomic characterization fo plasma-derived clotting factor VIII-von Wlllebrand factor concentrates, Electrophoresis (2009) 30, 3636-3646. Clifton, JC< Huang, F, Gaso-Sokac, D, Brilliant, K, Hixson, D, and Josic, Dj, Use of proteomics for validation of the isolation process of clotting factor IX from human plasma, J Proteomics (2010) 73, 678-688. Selected Publications Josic Dj and Zeilinger K, Membrane proteins, Methods in Enzymology (1996) 271: 113-134. Strancar A, Koselj P, Schwinn H and Josic Dj, Application of compact, porous disks for fast separation of biopolymers and in-process control in biotechnology, Analytical Chemistry (1996) 68: 3483-3488. Josi Dj, Kannicht Ch, Loester K, Pock K, Iberer G and Buchacher A, Vitronectin in clotting factor IX concentrates, Haemophilia (2001) 7: 250-257. Yang S, Lim Y-P, Zhou M, Salvemini P, Schwinn H, Josic Dj, Koo DJ, Chaudra IH and Wang P, Administration of human inter-a-inhibitors maintains hemodynamic stability and improves survival during sepsis, Critical Care Medicine (2002) 30: 617-622. Seanko E, Kannicht Ch, Loester K, Sarafanov A, Khrenov A, Kouiavskaia D, Shima M, Anayeva N, Schwinn H, Gruber G and Josic Dj, Development and applications of surface Plasmon resonance-based von Willebrand factor-collagen binding assay, Analytical Biochemistry (2002) 302: 252-262. Josic, Dj, Brown, MK, Huang, F, Lim Y-P, Rucevic, M, Clifton, JG, Hixson, DC, Proteomic characterization of inter-alpha inhibitor proteins from human plasma, Protemics (2006) 6, 2874-2885. Lawson, EL, Clifton, JG, Huang, F, Li X, Hixon, DC, Josic, Dj, Use of magnetic beads with immobilized monoclonal antibodies for isolation of highly pure plasma membranes, Electrophoresis (2006) 27, 2747-2758. Josic, Dj and Clifton, JG, Mammalian membrane proteins, Proteomics (2007) 7, 3010-3029. Josic, Dj, Clifton, JC, Kovac, S and Hixson, DC, Membrane proteins as diagnostic biomarkers and targets for new therapies, Current Opinion Molec Therapeutics (2008) 10, 116-123.
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Djuro Josic, PhD